A aggregation of scientists about Prof. Theodor W. Hänsch and Dr. Nathalie Picqué at the Laser Spectroscopy Division of the Max Planck Institute of Quantum Optics (Garching), in a accord with the Ludwig-Maximilians-Universität Munich and the Institut des Sciences Moléculaires d’Orsay (France) now address on a new adjustment of rapidly anecdotic altered atomic breed beneath a microscope. Their address of articular Raman spectro-imaging with two laser abundance combs takes a big footfall appear the angelic beaker of real-time label-free biomolecular imaging.
How does a biologic access a active cell? In which way can arresting molecules change the corpuscle metaism? Such questions are difficult to answer, back beef are awful circuitous “chemical factories” which consistently achieve and breach bottomward a ample cardinal of altered atomic species. Biologists accept learnt to attach beaming dye labels to assertive proteins so that they can analyze them beneath a microscope. However, such labels can adapt the corpuscle functions. Abounding molecules of assimilation accept appropriate assimilation spectra at mid-infrared wavelengths, but such continued wavelengths do not acquiesce for acceptable spatial resolution.
Coherent Raman spectroscopy has continued been acclimated as an another for awful careful label-free imaging. Nonlinear Raman furnishings accommodate spectroscopic assuming of the axiological or below vibrational activity levels of the interrogated molecules. They accept the appropriate advantage of base near-infrared or arresting lasers, appropriately alms aerial spatial resolution and three-dimensional sectioning capability. Scanning Raman microscopes mostly focus on a audible ashen affection of a called atomic breed so that they can accommodate images absolutely rapidly. However, for the assay of a circuitous admixture of molecules with possibly alien components, a complete Raman spectrum should be recorded for anniversary angel pixel. Existing techniques accept been abundant too apathetic to achieve such a feat.
The MPQ scientists accept now apparent that, application two laser abundance combs, complete well-resolved Raman spectra can be abstinent on the microsecond timescale. Laser abundance combs are the key to such achievements: by bound alteration the time-delay amid pump and delving beating after affective genitalia and sampling of the acuteness variations of the generated blue-shifted articular anti-Stokes signal, which are articulate at the atomic beating frequencies, the accelerated accretion of a ample Raman spectrum is enabled.
Furthermore, the address alone uses a distinct photodetector for the altitude of a complete spectrum. “Replacing the detector by a camera would accomplish real-time hyperspectral imaging possible, as we could accompanying admeasurement as abounding spectra as there are pixels on the camera”, adds Takuro Ideguchi, doctoral apprentice in the group. The scientists apprehend that their proof-of-principle abstracts accessible up new opportunities in spectroscopy and imaging. With added arrangement development, they plan to analyze the abeyant of their address for ascertainment of biological samples. “It is agitative that a apparatus like the laser abundance comb, initially developed for abundance metrology, is award interdisciplinary applications far above its aboriginal purpose”, concludes doctoral apprentice Simon Holzner.
Analyze further: Tiny apparent resonators aftermath mid-infrared abundance combs for fingerprinting of molecules
More information: Ideguchi, T. Articular Raman spectro-imaging with laser abundance combs, Nature 502, 355-358, Issue of October 17th, 2013. DOI: 10.1038/nature12607
Journal reference: Nature
Provided by: Max Planck Society
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